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KMID : 0380620130450030285
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Korean Journal of Food Science and Technology
2013 Volume.45 No. 3 p.285 ~ p.292
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Development and Validation of an Analytical Method for Ametoctradin Residue Determination in Domestic Agricultural Commodities by HPLC-PDA
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Do Jung-Ah
Kwon Ji-Eun
Lee Eun-Mi
Kim Mi-Ra
Kuk Ju-Hee
Cho Yoon-Jae
Kang Il-Hyun
Kim Hyung-Soo
Kwon Ki-Sung
Oh Jae-Ho
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Abstract
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This study was carried out to validate the safety of ametoctradin residues in agricultural commodities by developing an official analysis method. An analytical method was developed and validated using HPLC-PDA detectors. The samples were extracted with methanol, subsequently partitioned with dichloromethane and purified with florisil column chromatograph using acetone/hexane (30/70, v/v) as solvent. The method was validated by using grape, hulled rice, mandarin, and potato spiked with ametoctradin at 0.05 and 5.0 mg/kg, and pepper at 0.05 and 2.0 mg/kg. Average recoveries were 76-114.8% with relative standard deviation less than 10%, and the limit of detection and limit of quantification were 0.0125 and 0.05 mg/kg, respectively. The result of recoveries and overall coefficient of variation of the laboratory results from Gwangju regional Food and Drug Administration (FDA) and Daejeon regional FDA was accorded with Codex Alimentarius Commission Guideline (CAC/GL 40). Based on these results, this method was found to be appropriate for ametoctradin residue determination and can be used as the official method of analysis.
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KEYWORD
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ametoctradin, agricultural commodities, official method of analysis, HPLC-PDA, fungicide
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